Calculateur IC50 / EC50 4PL
Dose-réponse 4PL · IC50/EC50 · pente de Hill · R²
Calculateur IC50 / EC50 4PL
Dose-réponse 4PL · IC50/EC50 · pente de Hill · R²
Calcul local dans ce navigateurOrdre des colonnes: concentration, réponse
Tableau modifiable
| concentration | response | |
|---|---|---|
Coller CSV / TSV
Conversion: 1 µM = 1 µM
Résultat
Loading chart…
Data requirements
Saisissez au moins 4 lignes valides. Les concentrations doivent être positives et les réponses numériques.
concentration, réponseReview before reporting
Réservé à la recherche. Validez avec les données et le plan approuvé.
Check raw observations, excluded rows, model assumptions and study-specific acceptance criteria before using the result.
Transparent analysis
Inputs are processed locally in your browser. Review the calculation policy, limitations and verification approach before reporting a result.
Read the methodology →EVIDENCE & REVIEW
A result you can audit.
Reviewed 12 August 20264-parameter logistic workflow review complete
Implementation check
The workflow preserves raw rows, shows the fitted curve and reports model parameters so users can judge whether the observed concentrations bracket the response midpoint.
Interpretation boundary
An estimated IC50 is model- and assay-dependent. Sparse plateaus, constrained parameters, outliers or an unbracketed midpoint can produce a precise-looking but weak estimate.
Method references
Assay Operations for SAR SupportNCBI Assay Guidance ManualDose-response curve fitting, 4PL use and IC50 interpretation context.
REFERENCE VALIDATION CASE
Bracketed synthetic 4PL midpoint
Noise-free values generated from the same four-parameter logistic form used by the calculator.
Inputs
- Bottom 0, top 100
- Hill slope 1
- Synthetic midpoint at 1 µM
- Concentrations on both sides of 1 µM
Expected result
- Fitted midpoint approximately 1 µM
- Top and bottom approach 100 and 0
- R² greater than 0.999 for noise-free data
Scope: A software regression check, not biological or assay validation.
Review and correction history
12 August 2026 · Internal implementation review
4-parameter logistic workflow review complete. Formula behavior, unit handling, limitations and linked references were checked internally.
Independent reviewer: Not yet published. A name, relevant qualification, date and exact scope will appear here only after a real review is completed.
Source-informed and internally checked; independent scientific or regulatory validation is not claimed. Research use only.