Z′-factor Assay QC
Positive/negative controls · CV · signal window · Z′
Z′-factor Assay QC
Positive/negative controls · CV · signal window · Z′
Calculated locally in this browserColumn order: control_positive_or_negative, value
Editable data table
| control_positive_or_negative | value | |
|---|---|---|
Paste CSV / TSV
Analysis result
| Control | N | Mean | SD | CV % |
|---|---|---|---|---|
| Positive | 4 | 2.112 | 0.05123 | 2.425 |
| Negative | 4 | 0.2075 | 0.025 | 12.05 |
⚠ Z′ summarizes control separation and variability but is not a universal pass/fail rule; interpret it with assay purpose, effect size and hit-selection strategy.
Data requirements
Enter positive or negative control label and replicate value.
control_positive_or_negative, valueReview before reporting
Research use only. Validate raw data, exclusions and interpretation against the approved analysis plan.
Check raw observations, excluded rows, model assumptions and study-specific acceptance criteria before using the result.
Transparent analysis
Inputs are processed locally in your browser. Review the calculation policy, limitations and verification approach before reporting a result.
Read the methodology →