4PL dose-response · IC50/EC50 · Hill slope · R²
↗ RESEARCH WORKSPACE · 33 TOOLS · 6 LANGUAGES
From bench question
to clean result.
Plan experiments, prepare reagents, quantify assays and export clear figures in one focused workspace built for research.
01 Unit-aware02 Research checks03 Figure-ready04 Local processing
YOUR RESEARCH, ONE WORKSPACEStart with the task.
Start with the task.
Filter by the science.
Move through the research workflow or narrow by field. One tool can belong to several contexts without forcing you through duplicate menus.
Workflow
Research field
4PL/5PL standard fitting · unknown interpolation
↗AUC · Cmax · Tmax · t½ · CL/F · Vz/F
↗Censoring-aware survival · median · risk table · log-rank
↗Calculate C1V1 = C2V2 dilutions with unit conversion, pipetting volumes, DMSO carryover checks, and a bench-ready recipe.
↗Calculate cell suspension and medium volumes for plate seeding with viability correction, plate presets, and excess volume.
↗Scale per-reaction PCR or qPCR component volumes across reactions with configurable excess volume.
↗Calculate total cells/mL, viable cells/mL, and viability from hemocytometer counts with dilution correction.
↗CORE · COMPOUNDSDMSO-aware
Dilution
A dilution calculator that also catches impractical pipetting and solvent carryover.
BENCH RECIPE✓ READY
2µLstock
+998µLdiluent
Mix to 1 mL final volume. Calculated stock-solvent carryover: 0.2%.
- Pipette 2 µL of the 10 mM stock.
- Add 998 µL diluent and mix thoroughly.
500× dilution
FROM NUMBER → ACTIONMost calculators stop at the answer.
Most calculators stop at the answer.
Tossora Lab finishes the bench decision.
Every tool is designed around the moment after the math: what to pipette, how much to prepare, what might be impractical, and what belongs in the protocol.
01EnterExperimental conditions
→02CheckUnits + feasibility
→03PrepareBench-ready recipe
→04CopyProtocol-ready output
DESIGNED TO CATCH THE SECOND ERRORThe math can be right
Tossora Lab checks what a plain formula does not.The math can be right
and the experiment still be wrong.
Unit-aware by default
Mix nM, µM, mM, ng, µg, µL and mL without doing conversions in your head.
Bench-feasibility warnings
Flags sub-µL handling, solvent carryover and impossible mixtures before they reach the pipette.
Experimental context
Replicates, overage, viability, purity and plate presets are part of the calculation — not afterthoughts.
Private by design
Calculations run in your browser. No account is required, and experimental inputs are not submitted to a Tossora Lab database.
FORMULAS & ASSUMPTIONS
Know exactly what the calculator is doing.
Tossora Lab exposes the core equation behind each workflow and adds practical checks around the math. These tools support research planning; your approved protocol, reagent specification and instrument guidance remain authoritative.
Dilution & treatment
C₁V₁ = C₂V₂Concentrations and volumes are normalized to base units before solving. Solvent carryover is calculated from the stock fraction.
Molarity & stock
n = m / MWMoles are derived from mass and molecular weight; stock preparation can correct the weighed mass for entered assay/purity.
Cell seeding & counting
cells ÷ viable densitySeeding uses viable density; hemocytometer counting makes the chamber factor and dilution correction explicit.
DNA/RNA & protein
copies = mol × NₐCopy number, A260 concentration and protein molarity keep the conversion assumptions visible next to the result.
Centrifugation
RCF = 1.118×10⁻⁵ × r × RPM²Rotor radius is expressed in centimeters. Always use the actual rotor radius specified for your centrifuge setup.
qPCR relative expression
fold = (1 + E)⁻ΔΔCtEfficiency-aware comparative Ct requires a suitable reference assay and a predefined analysis plan.
QUICK ANSWERS
Before you use Tossora Lab at the bench.
Does Tossora Lab save my experimental inputs?
No Tossora Lab account or hosted experiment database is used by these calculators. Calculations run in the browser.
Can I use the generated recipe as my protocol?
Use it as a calculation and planning aid. Verify critical values, material specifications, route limits and procedures against your approved protocol and institutional requirements.
Why does Tossora Lab warn about tiny pipetting volumes?
A mathematically correct dilution can still be impractical at the bench. Tossora Lab surfaces small-volume and solvent-carryover risks so you can redesign the preparation before dosing.