PCR / qPCR Master Mix 計算機
1反応あたりの組成を反応数と余剰分に合わせてスケールします。
PCR / qPCR Master Mix 計算機
反応数と余剰率からprimer、master mix、water、templateの総量を計算します。
計算する ↓基本式
component × reactions × excessMOLECULAR
PCR / qPCR Master Mix 計算機
反応数と余剰率からprimer、master mix、water、templateの総量を計算します。
計算結果✓ 完了
成分1反応あたり調製総量
Master mix / 反応10 µL264 µL
Forward primer / 反応0.8 µL21.12 µL
Reverse primer / 反応0.8 µL21.12 µL
Nuclease-free water6.4 µL169 µL
Template / 反応2 µL52.8 µL
総調製量: 528 µL.
26.4 rxn-eq
主な用途
- qPCR plate用master mix調製
- 反応数に応じた試薬量の換算
- pipetting loss用の余剰分追加
使用前の確認
研究用途のみ。重要な計算値と実験条件は、承認済みプロトコルおよび施設要件で確認してください。
EVIDENCE & REVIEW
A result you can audit.
Reviewed 12 August 2026Reaction-scaling and overage review complete
Implementation check
Each per-reaction component is scaled by the reaction count and user-defined overage, while component totals and final prepared volume remain visible for review.
Interpretation boundary
The calculator scales an entered assay recipe; it does not validate primer concentration, chemistry, cycling conditions, inhibition, efficiency or reference-gene suitability.
Method references
The MIQE guidelinesClinical Chemistry / PubMedMinimum information for transparent and reproducible qPCR experiments.
REFERENCE VALIDATION CASE
Reaction scaling with overage
Per-reaction component volumes multiplied by reactions × 1.10.
Inputs
- 20 µL per reaction
- 10 reactions
- 10% overage
Expected result
- 11 reaction-equivalents
- 220 µL prepared total
- Every component scaled by 11
Scope: Checks scaling, not assay chemistry or cycling conditions.
Review and correction history
12 August 2026 · Internal implementation review
Reaction-scaling and overage review complete. Formula behavior, unit handling, limitations and linked references were checked internally.
Independent reviewer: Not yet published. A name, relevant qualification, date and exact scope will appear here only after a real review is completed.
Source-informed and internally checked; independent scientific or regulatory validation is not claimed. Research use only.
よくある質問
なぜ余剰分を加えますか？
分注時のdead volumeやpipetting lossを補うためです。
Templateも混ぜますか？
実験設計によります。sampleごとに別添加する場合は別途分注してください。