Plan serial dilution concentrations and transfer volumes with a visual plate-oriented workflow.
Calculate now ↓C₁V₁ = C₂V₂ at each stepGenerate the full concentration ladder and preserve a defined working volume at intermediate steps.
Add 200 µL diluent to each receiving tube; transfer 100 µL forward after thorough mixing.
Build a reproducible concentration series while keeping stepwise transfer and diluent volumes explicit.
Tossora Lab keeps units, intermediate values, and practical preparation checks visible so a mathematically correct answer is easier to translate into an experimental workflow.
Research use only. Verify critical calculations, reagent specifications, route or instrument limits, and procedures against your approved protocol and institutional requirements.
Define the starting concentration, dilution factor, number of steps and working volume. Review every concentration and transfer before starting, then mix each step completely before using it to prepare the next dilution.
Try it with your values ↑A serial dilution assumes the concentration of each source step is uniform. Incomplete mixing can propagate error through every downstream dilution.
No. Serial dilution uses each prepared step as the source for the next, whereas direct dilution independently prepares each target from the original stock.